| 摘要 |
Proteolytic enzymes are separated from proteases by a process wherein the protease solution is subjected one or more times to an extractive cycle comprising the steps of (a) contacting the solution with a carboxymethylpoly-saccharide ion-exchanger at a pH of 2.5 to 8.5, (b) separating the ion-exchanger from the solution thus treated and (c) eluating proteolytic enzyme from the ion-exchanger with an eluating solution buffered to a pH value higher than that at which the enzyme was adsorbed. The ion-exchanger is preferably a carboxymethylcellulose or a carboxymethyldextran ion-exchanger and the eluating solution is preferably a phosphate buffer. When the solution is subjected to more than one extraction cycles the pH in the second and subsequent cycles is preferably lower than that in preceding cycles. The process is particularly applicable to the production of enzymes designated Protease I, II and III from a protease solution obtained by treating with tannin a filtered culture medium obtained from a submerged cultivation of Aspergillus oxyzae until the onset of the spore formation to precipitate out the proteases and other proteins, separating the precipitate, treating the precipitate with acetone to extract the acetone-soluble constituents, and dissolving the acetone-insoluble constituents in an aqueous medium.ALSO:Pharmaceutical compositions contain a protease designated protease I or a mixture of proteases selected from proteases designated proteases I, II and III, which proteases rapidly decompose clotted blood, transudates and necrotic tissues, and are obtained by isolation from the protease solution obtained by cultivation of Aspergillus oryzae (see Division C3). The protease may be admixed with an injectable pharmaceutical diluent to produce an injectable solution which may also contain heparin, or with a water-free fatty ointment base or water-free pharmaceutical diluent in powder form. The ointment or powder may also contain a buffer system which when brought into contact with body fluids controls the pH of the ointment or powder and an antibiotic such as a penicillin, a tetracycline, tyrothricin, kanamycin, neomycin, bacitracin, polymyxin, trichomycin or xanthocillin, or a mixture of two or more of these antibiotics.
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