发明名称 |
NUCLEASE ACTIVITY OF TAL EFFECTOR AND FOKI FUSION PROTEIN |
摘要 |
<p>The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding domain and a cleavage domain. The cleavage domain can be from any endonuclease. In certain embodiments, the endonuclease is a Type IIS restriction endonuclease. In further embodiments, the Type IIS restriction endonuclease is FokI.</p> |
申请公布号 |
EP2580331(A1) |
申请公布日期 |
2013.04.17 |
申请号 |
EP20110796103 |
申请日期 |
2011.02.11 |
申请人 |
IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. |
发明人 |
YANG, BING;LI, TING;HUANG, SHENG |
分类号 |
C12N15/62;C07K19/00;C12N15/52;C12R1/64 |
主分类号 |
C12N15/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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