PROCESS OF GENOTYPING OF STAPHYLOCOCCUS AUREUS

摘要

The present invention relates to a method for genotyping the Staphylococcus aureus species by MLVA, comprising the steps consisting in: (i) carrying out a co-amplification of at least ten VNTR loci by multiplex PCR in a reaction medium comprising a DNA sample resulting from a Staphylococcus aureus isolate in order to form a plurality of amplicons for each of the ten VNTR loci; (ii) analysing said amplicons by capillary electrophoresis in order to obtain an electrophoretic profile of said sample; and (iii) assigning a numerical MLVA code to said electrophoretic profile. The present invention also relates to a Staphylococcus aureus genotyping kit for carrying out the above-mentioned genotyping method.