发明名称 |
Method for continuously detecting glucose concentration in sample, kit thereof and method for using biosensor |
摘要 |
The invention provides a method for continuously detecting glucose concentration in a sample, including: (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution. |
申请公布号 |
US8415170(B2) |
申请公布日期 |
2013.04.09 |
申请号 |
US20100717542 |
申请日期 |
2010.03.04 |
申请人 |
LEE KUN-FENG;CHEN CHIEN-AN;CHANG HONG-WEN;LIN YUH-JIUAN;INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE |
发明人 |
LEE KUN-FENG;CHEN CHIEN-AN;CHANG HONG-WEN;LIN YUH-JIUAN |
分类号 |
G01N33/543 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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