MEDIUM WITH THE WATER-EXTRACT OF ZIZYPHUS JUJUBA MILLER FOR FERMENTATION OF BIFIDOBACTERIUM

摘要

PURPOSE: A medium for fermenting Bifidobacterium sp. is provided to control growth of intestinal beneficial bacteria and to be used as a medium for growing probiotics. CONSTITUTION: A medium for fermenting Bifidobacterium sp. contains a hot water extract of jujube, which is prepared by adding hot water to jujube, extracting, and removing residues. The Bifidobacterium sp. includes Bifidobacterium bifidum or Bifidobacterium adolescentis. [Reference numerals] (AA) Primary extract; (BB,JJ) Jujube(Boeun-jujube, 20g); (CC,QQ) Adding 20 times distilled water into jujube; (DD,RR) Adding 20 times 99.9% ethanol into jujube; (EE) Stirring(25°C, 200rpm, 24hours, 1time); (FF,MM) Vacuum filtration(filtering paper, whatman No.3); (GG) Concentration(40°C); (HH) Constant volumizing(50mL); (II) Secondary extract; (KK) Adding 3.5 times distilled water into jujube; (LL) Stirring(25°C, 200rpm, 24hours); (NN) Extraction; (OO) Removal; (PP) Remaining product; (SS) Identical to the primary extract manufacturing process