METHOD AND APPARATUS FOR GENERATING THERMAL MELTING CURVES IN MICROFLUIDIC DEVICE

摘要

PROBLEM TO BE SOLVED: To provide novel methods and devices that employ microfluidic technology to generate molecular melt curves.

SOLUTION: A method of determining the value of a physical parameter that corresponds to a reference temperature within a channel in a microfluidic device includes: flowing a fluid comprising a molecule of known Tmthrough the channel; varying a physical parameter that correlates with the temperature within the channel; generating a thermal property curve for the molecule by measuring the value of a detectable property of the molecule as a function of the parameter; and determining the values of the detectable property and the parameter at the point in the thermal property curve that corresponds to the Tmof the molecule.

COPYRIGHT: (C)2013,JPO&INPIT