MULTILAYER TEST PIECE FOR ASSAYING GLYCOSYLATED HEMOGLOBIN AND ASSAY METHOD USING SAME

摘要

[Problem] To provide a multilayer test piece for colorimetrically assaying glycosylated hemoglobin, said multilayer test piece being little affected by glycosylated amino acids and/or glycosylated peptides, which possibly exist in a test sample and are not derived from glycosylated hemoglobin, and having good storage stability, and an assay method. [Solution] A multilayer test piece for colorimetrically assaying glycosylated hemoglobin, characterized in that said multilayer test piece comprises at least the following layer (a) and layer (b) that are laminated in the order of layer (a)/layer (b) from the surface on which a test sample is to be spotted, and said multilayer test piece has no blood separation layer: layer (a): a polymer base material carrying at least glycosylated amino acid oxidase; and layer (b): a polymer base material carrying at least a protease.