| 摘要 |
<p>The purpose of the present invention is to produce a protein by efficiently culturing plant cells while preventing the inhibition of translation in mRNA of the plant cells that are cultured, in particular, under stress conditions due to the lack of a factor essentially required for the growth (for example, nutrient-starvation stress or anoxic stress). Provided are a method, said method comprising a step for culturing plant cells, which have been transformed by a recombinant DNA molecule encoding mRNA having a 5'UTR(a) or (b) as mentioned below, under stress conditions due to the lack of a factor essentially required for the growth, to thereby prevent the inhibition of translation of a protein encoded by the aforesaid mRNA by the stress due to the lack of the factor essentially required for the growth, and so on: (a) a 5'UTR comprising a base sequence represented by SEQ ID NO:1, 2, 3, 4, 5 or 6; and (b) a 5'UTR comprising a base sequence, which is derived from the base sequence of 5'UTR(a) by substitution, deletion or addition of one to several bases, and being capable of preventing the inhibition of translation by the stress due to the lack of the factor essentially required for the growth.</p> |
