发明名称 |
METHOD OF PREPARING PLANT-DERIVED VLPS |
摘要 |
Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction. |
申请公布号 |
EP2480658(A4) |
申请公布日期 |
2013.02.27 |
申请号 |
EP20100818190 |
申请日期 |
2010.09.21 |
申请人 |
MEDICAGO INC. |
发明人 |
VEZINA, LOUIS-PHILIPPE;COUTURE, MANON;PAQUET, DANY;DARGIS, MICHELE;D'AOUST, MARC-ANDRE |
分类号 |
C07K1/14;C07K1/34;C07K14/415;C12N5/04;C12N15/00;C12N15/82 |
主分类号 |
C07K1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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