METHOD FOR PREPARING ACTIVE FORM OF TNFR-FC FUSION PROTEIN

摘要

PURPOSE: A method for isolating and preparing a TNFR(Tumor Necrosis Factor Receptor)-Fc fusion protein using hydrophobic interaction chromatography(HIC) is provided to isolate high purity active proteins. CONSTITUTION: A method for preparing an active TNFR-Fc fusion protein comprises: a step of injecting a sample containing a mixture solution of the TNFR-Fc fusion protein prepared from mammalian cells into a HIC column which is pre-equilibrated using an equilibration buffer containing sodium citrate, sodium sulfate, or sodium phosphate; a step of washing the column with a wash buffer; and a step of eluting the active TNFR-Fc fusion protein from the column using an elution buffer.