METHOD FOR DETECTION OF TRANSFORMATION EVENT OF MAIZE MON810 BY MULTIPLEX POLYMERASE CHAIN REACTION

摘要

A method for detection of transformation event of maize MON810 in genetically modified plant by multiplex polymerase chain reaction, for realization of which denaturation of plant DNA is carried out; cycles each of which comprises DNA denaturation, renaturation of plant DNA with oligonucleotide primers, synthesis of target genes fragments; final synthesis of fragments of target genes. For carrying out of multiplex polymerase chain reaction the pair of oligonucleotide primers is used, which are specific to transformation event MON810 (0.25 μm VM01; 0.25 μm VM03), to genetic construction in which the transformation was carried out (0.45 MicMmgl; 0.45 μm mg2),and pair of primers for fragment of own maize gene adh-1 (0.20 μm Adh-F3; 0.20 μm Adh-Rl).