发明名称 |
PREPARATION OF MAYTANSINOID ANTIBODY CONJUGATES BY A ONE-STEP PROCESS |
摘要 |
The invention provides a one-step process for preparing a cell-binding agent cytotoxic agent conjugate comprising contacting a cell-binding agent with a cytotoxic agent to form a first mixture comprising the cell-binding agent and the cytotoxic agent and contacting the first mixture comprising the cell-binding agent and the cytotoxic agent with a bifunctional crosslinking reagent, which provides a linker, in a solution having a pH of about 4 to about 9 to provide a second mixture comprising the cell-binding agent cytotoxic agent conjugate, wherein the cell-binding agent is chemically coupled through the linker to the cytotoxic agent, free cytotoxic agent, and reaction by-products. The second mixture is then optionally subjected to purification to provide a purified cell-binding agent cytotoxic agent conjugate. |
申请公布号 |
WO2012135517(A3) |
申请公布日期 |
2013.02.21 |
申请号 |
WO2012US31243 |
申请日期 |
2012.03.29 |
申请人 |
IMMUNOGEN, INC.;LI, XINFANG;WORFUL, JARED M. |
发明人 |
LI, XINFANG;WORFUL, JARED M. |
分类号 |
A61P35/00;A61K47/48 |
主分类号 |
A61P35/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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