| 摘要 |
Provided is photometric analysis technique that, in a scanning molecule counting method in which the light of light emitting particles in a sample solution is detected using a confocal microscope or a multiphoton microscope, reduces as much as possible the possibility that identical light emitting particles are detected as different particles, and that, in an embodiment in which the dimensions and shape of a light detection region change as little as possible, enables scanning in a sample solution by moving the light detection region along a wider region or a path having longer distance. In this photometric analysis technique, in the sample solution, while the position of a light detection region is moved along a second path a position of which moves along a first path, the light from a light detection region is detected and time-sequence light intensity data is generated, and using the time-sequence light intensity data, signals that indicate the light from light emitting particles within a prescribed path are individually detected. |
