MICROSCOPE APPARATUS FOR DETECTING OR IMAGING PROTEIN USING IFRET PROBE, AND METHOD FOR DETECTING OR IMAGING PROTEIN USING SAME

摘要

A microscope apparatus for detecting or imaging a target protein using an induced fluorescence resonance energy transfer (iFRET) probe, according to the present invention, comprises: a light irradiation portion for irradiating a first light within a wavelength which excites an amino acid inside the target protein, and a second light within a wavelength which excites a fluorescent molecule in the iFRET probe; an objective lens for incidenting the light which is irradiated from the light irradiation portion on a sample into which the iFRET probe is introduced; and a recognition portion for detecting a first light emission signal, which is generated from the iFRET probe by irradiating on the sample the first light within the wavelength that excites the amino acid inside the target protein, and a second light emission signal, which is generated from the iFRET probe by irradiating the second light within the wavelength that excites the fluorescent molecule in the iFRET probe, wherein the iFRET probe is comprised by binding directly or through a linker a target protein-specific binding site or a molecule having the binding site, and a fluorescent molecule having an acceptor function to the intrinsic fluorescence of the target protein.