LYSOPHOSPHATIDIC ACID FROM GINSENG AND MANUFACTURING METHOD THEREOF

摘要

PURPOSE: A method for preparing lysophosphatidic acid(LPA) isolated and identified from ginseng is provided to ensure a physiological and pharmacological action and to enhance stability. CONSTITUTION: Lysophosphatidic acid(LPA) is isolated and identified from ginseng. LPA is contained in edible crude gintonin isolated and identified from ginseng using ethanol. LPA forms a complex through a hydrophobic bond of the sugar of the crude gintonin and proteins. A method for preparing LPA isolated and identified ginseng comprises: a step of extracting ginseng with ethanol and preparing an ethanol extract; a step of concentrating, dialyzing, and freeze-drying the ethanol extract; a step of dissolving the ethanol extract in an ethanol-containing buffer solution and performing anion exchange resin chromatography; a step of dialyzing materials collected by an anion exchange resin column and removing salts and ethanol to obtain the crude gintonin; and a step of performing the analysis and quantitation of LPA through LC-ESI-MS/MS of the crude gintonin.