摘要 |
PURPOSE: A method for preparing 2-pyrrolidone using biomass is provided to enable industrial mass production through simplified processes. CONSTITUTION: A method for preparing 2-pyrrolidone comprises: a step of culturing a microorganism containing glutamate decarboxylase in a medium glutamic acid or glutamate to prepare 4-amino butyric acid; a step of treating the medium with activated charcoal and removing colorant from the medium; a step of filtering and collecting 4-amino butyric acid from the medium; and a step of converting 4-amino butyric acid into 2-pyrrolidone. The microorganism is pretreated with hydrophobic organic solvent. |