Gradient elution electrophoresis and detectorless electrophoresis apparatus

摘要

A microfluidic apparatus and method for performing electrophoretic separation of compounds. The method comprises the steps of: a) providing a separation buffer; b) providing a sample solution in fluid contact with the separation buffer; c) applying an electric field to the separation buffer; and d) producing a variable bulk flow of the separation buffer in a direction substantially aligned with said electric field. Fluid contact between the separation buffer and the sample solution is made through a separation column having a length in the range of from approximately 0.01 mm to approximately 5 mm. By the foregoing, compounds can be sequentially detected and quantified.