Fusion Collagenase in which affinity TAG is linked and method for producing the same

摘要

593239 Disclosed is a method for producing a fusion collagenase said method comprising: culturing E. coli which are transformed by a DNA encoding a fusion collagenase; and purifying the culture of step (a) using an affinity column to selectively collect the fusion collagenase which has a collagen-binding domain, wherein the fusion collagenase comprises: (i) an amino terminal of an affinity tag which is directly or indirectly linked to a carboxy terminal of a collagenase; (ii) said collagenase and said affinity tag are linked to each other in such a manner that a fragment having collagenase activity and the affinity tag are separated from each other when the fusion collagenase expressed in the E. coli is degraded by the action of a protease; and (iii) said affinity tag is bound by said affinity column.