IMPROVED <i>E. COLI</i> EXTRACT FOR PROTEIN SYNTHESIS
摘要
There is now a market for kits containing the necessary components for performing protein synthesis reactions using template DNA of the experimenter. One category of existing system for performing protein synthesis reactions is based on an S-30 extract from the bacteria <i>E. coli</i>. It is disclosed here that a simple fractionation process can dramatically improve the performance of an S-30 prokaryotic protein synthesis reaction mixture. In one embodiment, the fractionation is a simple freezing and thawing of an S-30 extract combined with a supplemental mix, followed by centrifugation. The resulting fractionated S-30 reaction mixture yields more full-length target protein and lessnon-full length or non-target protein than possible using prior art S-30 systems.
申请公布号
WO0183805(A2)
申请公布日期
2001.11.08
申请号
WO2001US14469
申请日期
2001.05.03
申请人
NOVAGEN, INC.
发明人
AMBUEL, YUPING;VAN OOSBREE, THOMAS, R.;MCCORMICK, MARK, R.;MIERENDORF, ROBERT, M.