发明名称 |
A SYNTHETIC HEPATITIS C GENOME AND METHODS OF MAKING AND USE |
摘要 |
Synthetic representative HCV subtypes, including a 1a and 1b genome, dubbed Bole1a and Bole1b, are provided using an inventive method of Bayesian phylogenetic tree analysis, ancestral sequence reconstruction and covariance analysis. Bole1a branches centrally among 390 full-genome sequences used in its design, a carefully curated 143 sequence full-genome dataset, and separate genomic regions including an independent set of 214 E1E2 sequences from a Baltimore cohort. Bole1a is phylogenetically representative of widely circulating strains. Full genome non-synonymous diversity comparison and 9-mer peptide coverage analysis showed that Bole1a is able to provide more coverage (94% and 78% respectively) than any other sequence in the dataset including H77, a traditional reference sequence. Bole1a also provides unsurpassed epitope coverage when compared to all known T cell epitopes. |
申请公布号 |
WO2012151263(A3) |
申请公布日期 |
2013.01.17 |
申请号 |
WO2012US36098 |
申请日期 |
2012.05.02 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY;RAY, STUART, CAMPBELL;MUNSHAW, SUPRIYA;LIU, LIN |
发明人 |
RAY, STUART, CAMPBELL;MUNSHAW, SUPRIYA;LIU, LIN |
分类号 |
C12N15/33;A61K39/12;C12N5/10;C12N15/11;C12Q1/68;G01N33/569 |
主分类号 |
C12N15/33 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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