Method of making a multi-layer pad

摘要

A method of making a multi-layer pad for use in determining an analyte concentration, the method comprising the acts of:
immersing a first layer of the multi-layer pad in a first solution including at least one enzyme, the first layer having a first surface and a second surface;
removing the first layer from the first solution;
drying the first layer such that the at least one enzyme is physically absorbed in the first layer;
after drying the first layer, applying a second solution to at least one of the first surface and the second surface of the first layer; and
chemically cross-linking the second solution to the first layer, the chemically cross-linked second solution forming at least one outer polymeric layer.