发明名称 |
System and method for detection of HIV tropism variants |
摘要 |
An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is described that comprises the steps of: generating cDNA species from each RNA molecule in an HIV sample population; amplifying at least one first amplicon from the cDNA species, wherein each first amplicon comprises a plurality of amplified copies and is amplified with a pair of nucleic acid primers that define a locus of the first amplicon; clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons wherein a plurality of the second amplicons comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition of the substantially identical copies from at least 100 of the immobilized populations in parallel on a single substrate; and detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV tropism.
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申请公布号 |
US8344123(B2) |
申请公布日期 |
2013.01.01 |
申请号 |
US20100970036 |
申请日期 |
2010.12.16 |
申请人 |
454 LIFE SCIENCES CORPORATION;SIMEN BIRGITTE BINDERUP;ST. JOHN ELIZABETH PATRICIA |
发明人 |
SIMEN BIRGITTE BINDERUP;ST. JOHN ELIZABETH PATRICIA |
分类号 |
C07H21/04;C12P19/34;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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主权项 |
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地址 |
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