MICROORGANISMS FOR PRODUCING 6-AMINOCAPROIC ACID

摘要

The invention provides a non-naturally occurring microbial organism having an adipate, a 6-aminocaproic acid, caprolactam, or hexametheylenediamine pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine pathway and at least one exogenous nucleic acid that encodes an enzyme that increases the yields of adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine by (i) enhancing carbon fixation via the reductive TCA cycle, and/or (ii) accessing additional reducing equivalents from gaseous carbon sources and/or syngas components such as CO, C02, and/or H2. The invention additionally provides a method for producing adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine. The method can include culturing an adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism described herein under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine.