ATTENUATED RECOMBINANT ALPHAVIRUSES INCAPABLE OF REPLICATING IN MOSQUITOES AND USES THEREOF

摘要

Disclosed is an attenuated, recombinant alphavirus comprising; an alphavirus nucleic acid, having: an inactivated subgenomic promoter; and an insertion of an internal ribosomal entry site of encephalomyelocarditis virus (EMCV IRES) between the end of nonstructural protein 4 (nsP4) coding sequence and initiating AUG of a subgenomic RNA coding sequence of the alphavirus, wherein the alphavirus is attenuated.