发明名称 |
METHOD FOR PRODUCING PREPARATION OF RECOMBINANT AAV VIRION SUBSTANTIALLY FREE OF EMPTY CAPSID |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide new methods for enhancing manufacturing capability by removing empty capsids from stocks of AAV vector particles or reducing the numbers of empty capsids. <P>SOLUTION: The empty capsids can be separated from rAAV virions ("AAA vector particles") containing genetic material using column chromatography techniques. Methods for separating AAV empty capsids from mixtures of AAV vector particles and AAV empty capsids are described. The methods use column chromatography techniques and provide for commercially viable levels of recombinant AAV virions. <P>COPYRIGHT: (C)2013,JPO&INPIT |
申请公布号 |
JP2012235788(A) |
申请公布日期 |
2012.12.06 |
申请号 |
JP20120170066 |
申请日期 |
2012.07.31 |
申请人 |
GENZYME CORP |
发明人 |
QU GUANG;WRIGHT JOHN FRASER |
分类号 |
C12N7/00;C12N;C12N7/02;C12N15/09;C12N15/86;C12N15/864 |
主分类号 |
C12N7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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