| 摘要 |
<p>PURPOSE: A primer or primer set for distinguishing 10 Brucella species is provided to distinguish B. canis, B. ceti, and B. microti from other similar bacteria and to diagnose and detect diseases. CONSTITUTION: A primer for distinguishing Brucella canis is denoted by sequence number 5. A primer of distinguishing Brucella ceti is denoted by sequence number 6. A primer set for distinguishing Brucella microti is denoted by sequence numbers 11 and 12. A single tube-multiplex PCR primer set for distinguishing Brucella comprises: a primer set of sequence numbers 1 and 2, 3 and 4, 5 and 6, 7 and 8, 9 and 10, 11 and 12, 13 and 14, 15 and 16, or 17 and 18. The Brucella includes Brucella abortus, Brucella canis, Brucella suis, Brucella ovis, Brucella meotomae, Brucella melitensis, Brucella ceti, Brucella pinnipedialis, Brucella microti, or Brucella inopinata.</p> |
