| 摘要 |
PURPOSE: A method for visualizing a primo vessel in a spinal cord is provided to efficiently visualize the primo vessel in the spinal cord by directly injecting samples to the spinal cord using intrathecal injection. CONSTITUTION: A sample is injected with in-situ and in-vivo(S601). Laminectomy is performed to remove a part of a vertebral arch which surrounds a spinal cord(S602). The spinal cord without the part of the vertebral arch is firstly observed by a microscope(S603). The nucleus of the primo vessel is dyed with DAPI(4',6-diamidino-2-phenylindole)(S604). A sample dyed with DAPI is secondly observed by a fluorescent microscope(S605). The primo vessel in the spinal vessel is thirdly observed with high magnification(S606). [Reference numerals] (AA) Start; (BB) Finish; (S601) Sample injecting process; (S602) Laminectomy; (S603) First observation; (S604) Dyeing process; (S605) Second observation; (S606) Third observation
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