发明名称 |
CHEMICALLY MODIFIED MUTANT SERINE HYDROLASES SHOW IMPROVED CATALYTIC ACTIVITY AND CHIRAL SELECTIVITY |
摘要 |
This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside. |
申请公布号 |
US2012295329(A1) |
申请公布日期 |
2012.11.22 |
申请号 |
US201213561257 |
申请日期 |
2012.07.30 |
申请人 |
JONES JOHN BRYAN;DICKMAN MICHAEL;LLOYD RICHARD C.;THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO |
发明人 |
JONES JOHN BRYAN;DICKMAN MICHAEL;LLOYD RICHARD C. |
分类号 |
C12N9/14;C12N9/48;C12N9/16;C12N9/50;C12N9/54;C12N9/78;C12P41/00 |
主分类号 |
C12N9/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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