发明名称 |
BACTERIAL MRNA SCREEN STRATEGY FOR NOVEL PESTICIDE-ENCODING NUCLEIC ACID MOLECULE DISCOVERY |
摘要 |
Methods are provided for isolating and identifying novel pesticide-encoding nucleic acid molecules from bacterial strains identified as having a pesticidal polypeptide. The methods involve selecting a bacterial strain having or suspected of having at least one pesticide-encoding plasmid, curing the at least one pesticide-encoding plasmid from the bacterial strain and performing a subtractive hybridization to obtain plasmid mRNA encoding the pesticidal polypeptide. The plasmid mRNA can be used to make a cDNA library from which the pesticidal polypeptide can be isolated and subsequently identified. |
申请公布号 |
CA2834364(A1) |
申请公布日期 |
2012.11.08 |
申请号 |
CA20122834364 |
申请日期 |
2012.04.30 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
ABAD, ANDRE R.;KAPKA-KITZMAN, DEIRDRE M. |
分类号 |
C12N15/82 |
主分类号 |
C12N15/82 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|