发明名称 ENZYMATIC EVOLUTION METHOD USING UNILAMELLAR LIPOSOME
摘要 <P>PROBLEM TO BE SOLVED: To improve screening efficiency in molecular-evolutional engineering technique (for example, enzymatic evolution). <P>SOLUTION: There is provided unilamellar liposomes such as a nuclease-treated unilamellar liposome comprising: (a) a DNA comprising a promoter sequence, a translational initiator sequence, and a polypeptide-encoding sequence; (b) an RNA polymerase; (c) a ribonucleotide; and (d) an acellular protein synthesis system, or a nuclease-treated unilamellar liposome comprising (a) an RNA comprising a translational initiator sequence and a polypeptide-encoding sequence, and (d) an acellular protein synthesis system. <P>COPYRIGHT: (C)2013,JPO&INPIT
申请公布号 JP2012210170(A) 申请公布日期 2012.11.01
申请号 JP20110076727 申请日期 2011.03.30
申请人 JAPAN SCIENCE & TECHNOLOGY AGENCY 发明人 YOMO TETSUYA;ICHIHASHI IKIKAZU;SUNAMI TAKESHI;NISHIKAWA TAKEHIRO
分类号 C12N15/09;C12M1/00;C12Q1/68;C40B40/06 主分类号 C12N15/09
代理机构 代理人
主权项
地址