RECOMBINANT HYBRID POLYPEPTIDE, CAPABLE OF INHIBITING HUMAN ENDOTHELIAL CELL PROLIFERATION IN VITRO, AND METHOD FOR PRODUCTION THEREOF

摘要

FIELD: chemistry. ^ SUBSTANCE: hybrid polypeptide is a polypeptide 1 to which a polypeptide 2 is covalently bonded, where polypeptide 1 is a human endostatin sequence with 135-184 amino acid residues, wherein at position 173, Cis is replaced with Ala with a relatively native endostatin sequence, and polypeptide 2 is a human plasminogen sequence with 82-341 or 463-511 amino acid residues. Also disclosed is a method of obtaining the hybrid polypeptide using an E.coli producer, which involves methods for extraction and purification thereof. ^ EFFECT: polypeptide is capable of inhibiting human endothelial cell proliferation in vitro and can be used when producing nontoxic preparations for inhibiting angiogenesis. ^ 4 cl, 4 dwg, 6 ex