METHOD AND COMPOSITIONS FOR SPECIFICALLY DETECTING PHYSIOLOGICALLY ACCEPTABLE POLYMER MOLECULES

摘要

Disclosed is a method for determining the number of physiologically acceptable polymer molecules bound to a protein or protein complex in a polymer-protein conjugate, comprising the steps of detecting binding between: (i) a polymer:protein conjugate having one or more polymers bound to the protein; and (ii) an antibody that specifically binds said polymer, said antibody detectable when bound to said polymer:protein conjugate, wherein the number of polymers in the polymer:protein conjugate correlates with levels of antibody detected bound to the polymer:protein conjugate when compared to a known control. Further disclosed is a method for determining the number of physiologically acceptable polymer molecules bound to a protein or a protein complex comprising: contacting said polymer with an antibody that specifically binds said polymer, said antibody detectable when bound to said polymer, wherein the number of polymers bound by the antibody correlates with levels of antibody detected bound when compared to a known control.