Glycosylation of molecules

摘要

1. A method of producing an altered N-glycosylation form of a target protein or polypeptide, comprises:
a) providing a cell genetically engineered to comprise at least one modified N-glycosylation activity and introducing into the cell a nucleic acid encoding the target protein or polypeptide, wherein the cell produces the target protein or polypeptide in an altered N-glycosylation form, the cell being a Yarrowia lipolytica cell, an Arxula adeninivorans or a cell from a related species of dimorphic yeast; or
b) contacting the target protein or polypeptide with a cell lysate prepared from a cell genetically engineered to comprise at least one modified N-glycosylation activity, the cell being a Yarrowia lipolytica cell, an Arxula adeninivorans or a related species of dimorphic yeast;
wherein the at least one modified N-glycosylation activity comprises a deficiency in OCH 1 activity and the target protein or polypeptide is useful in the pharmaceutical, food, beverage, agriculture or chemical industries.