发明名称 |
METHOD FOR AMPLIFICATION OF TARGET NUCLEIC ACID |
摘要 |
A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two probe-complementary portions; (b) contacting said fragmented nucleic acid sample with a probe comprising two target fragment-complementary portions complementary to the probe-complementary portions of the target fragment; (c) rendering the fragmented nucleic acid sample single-stranded; (d) allowing the probe-complementary portions to hybridise with the target-fragment complementary portions; (e) if the probe in step (b) is not immobilised, immobilising the probe-target fragment hybrid on a solid phase via immobilisation moiety; (f) separating non-immobilised nucleic acid fragments from the solid phase; (g) contacting the solid phase with a ligase to ligate ligatable 5′ and 3′ ends of the target fragment whereby the target fragment is circularized; and (h) amplifying said circularized target fragment. |
申请公布号 |
EP2507386(A1) |
申请公布日期 |
2012.10.10 |
申请号 |
EP20100785082 |
申请日期 |
2010.12.03 |
申请人 |
HALO GENOMICS AB |
发明人 |
ERICSSON, OLOF;ISAKSSON, MAGNUS;JOHANSSON, HENRIK;ROOS, FREDRIK;FREDRIKSSON, SIMON;NILSSON, MATS |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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