发明名称 |
Immunoaffinity isolation of modified peptides from complex mixtures |
摘要 |
The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites. |
申请公布号 |
US2012244594(A9) |
申请公布日期 |
2012.09.27 |
申请号 |
US20070823775 |
申请日期 |
2007.06.28 |
申请人 |
RUSH JOHN;ZHANG HUI;ZHA XIANGMING;COMB MICHAEL J.;TAN YI;CELL SIGNALING TECHNOLOGY, INC. |
发明人 |
RUSH JOHN;ZHANG HUI;ZHA XIANGMING;COMB MICHAEL J.;TAN YI |
分类号 |
C12N11/00;B01D11/02;C07K1/14;C07K1/22;C07K16/00;C07K16/18;C07K16/40;C12S3/14;G01N30/72;G01N33/566;H01J49/26 |
主分类号 |
C12N11/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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