发明名称 |
COMPOSITIONS AND METHODS FOR PRODUCING CLOSTRIDIAL COLLAGENASES |
摘要 |
<p>The present invention provides a method for producing a drug product comprising a combination of highly purified collagenase I and collagenase II from Clostridium histolyticum. The method utilizes an improved medium for the cultivation of Clostridium histolyticum which includes a non-meat-derived (i.e., non-mammalian) peptone or vegetable peptone. The method includes one or more of: (1) reducing glucose content in the meat-free or vegetable-derived media; and (2) increasing the salt concentration in the meat-free or vegetable-derived media. Also provided is a drug product which includes collagenase I and collagenase II at an optimized fixed mass ratio, and which has a purity of greater than at least 95%.</p> |
申请公布号 |
WO2012125948(A1) |
申请公布日期 |
2012.09.20 |
申请号 |
WO2012US29492 |
申请日期 |
2012.03.16 |
申请人 |
BIOSPECIFICS TECHNOLOGIES CORP.;WEGMAN, THOMAS L.;YU, BO |
发明人 |
WEGMAN, THOMAS L.;YU, BO |
分类号 |
C12N1/20;A61K38/48;C12N9/52 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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