Compositions And Methods For Producing A Composition

摘要

Methods for producing CLTA4-Ig compositions, in particular a composition comprising an isolated population of CTLA4-Ig molecules from a liquid culture medium. Said methods comprising: (a) harvesting the liquid culture medium from a culture of mammalian cells expressing CTLA4-Ig molecules; (b) separating CTLA4-Ig molecules from cellular components; (c) separating CTLA4-Ig molecules from MCP- 1; (d) separating CTLA4-Ig dimers from high molecular weight aggregates; and (e) separating CTLA4-Ig molecules into two or more fractions, wherein at least one fraction has a greater molar ratio of sialic acid to CTLA4-Ig molecules compared to at least one other fraction. Separating techniques utilised include hydrophobic interaction, affinity and anion exchange chromatography. Compositions containing the compounds are also claimed.