发明名称 |
RECOMBINANT HOST CELLS HAVING AN INCREASE IN BUOYANT DENSITY |
摘要 |
Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.
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申请公布号 |
US2012214202(A1) |
申请公布日期 |
2012.08.23 |
申请号 |
US201113164990 |
申请日期 |
2011.06.21 |
申请人 |
BHALLA RITU;CHEN QI;CHENG QIONG;PANDEY NEERAJ;ROUVIERE PIERRE E.;RUEBLING-JASS KRISTIN;SACHAN ANNAPURNA;E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
BHALLA RITU;CHEN QI;CHENG QIONG;PANDEY NEERAJ;ROUVIERE PIERRE E.;RUEBLING-JASS KRISTIN;SACHAN ANNAPURNA |
分类号 |
C12P21/06;C12N1/21 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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地址 |
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