发明名称 |
Eukaryotic amadoriase having excellent thermal stability, gene and recombinant DNA for the eukaryotic amadoriase, and process for production of eukaryotic amadoriase having excellent thermal stability |
摘要 |
<p>The object is to overcome the defects associated with thermal stability in a conventional eukaryotic amadoriase and to provide a eukaryotic amadoriase having excellent thermal stability for use as an enzyme for clinical diagnosis of diabetes or an enzyme sensor. Disclosed are: a eukaryotic amadoriase which is prepared by introducing a mutation into DNA encoding a eukaryotic amadoriase derived from a microorganism belonging to the genus Coniochaeta or Eupenicillium so as to introduce a substitution into a specific amino acid residue in the eukaryotic amadoriase, thereby overcoming the defect associated with thermal stability; a gene or recombinant DNA for the eukaryotic amadoriase; and a process for production of a eukaryotic amadoriasehaving excellent thermal stability.</p> |
申请公布号 |
EP2020439(B1) |
申请公布日期 |
2012.08.08 |
申请号 |
EP20070741740 |
申请日期 |
2007.04.17 |
申请人 |
KIKKOMAN CORPORATION |
发明人 |
HIROKAWA, KOZO;ICHIYANAGI, ATSUSHI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/04;C12Q1/26 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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