METHOD OF DETERMINING INHIBITORS OF COAGULATION

摘要

PROBLEM TO BE SOLVED: To provide improved methods for determining anticoagulants, which use two components interacting owing to close spatial proximity and generating a detectable signal, in such a way that low molecular weight ligands which is not required to have two or more artificial residue for association with any of signaling components can be used.

SOLUTION: A method for determining anticoagulants includes: admixing an aliquot of a sample which is suspected of containing an anticoagulant with a defined amount of a proteolytically active coagulation factor and with a ligand which binds to the active site of the proteolytically active coagulation factor but is not cleaved by the factor at a peptide bond; and measuring a signal generated by a signal-producing system as a result of binding of the ligand to the activated coagulation factor.

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