发明名称 |
DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR |
摘要 |
Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product. |
申请公布号 |
US2012190068(A1) |
申请公布日期 |
2012.07.26 |
申请号 |
US201213355125 |
申请日期 |
2012.01.20 |
申请人 |
GUERTIN PATRICK M.;CROWELL JOSEPH D.;XCELLEREX, INC. |
发明人 |
GUERTIN PATRICK M.;CROWELL JOSEPH D. |
分类号 |
C12N7/02;C12P21/00 |
主分类号 |
C12N7/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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