IMMUNOASSAY FOR DIRECT DETERMINATION OF ANTIGEN CONTENT OF PRODUCTS COMPRISING ADJUVANT-COUPLED-ANTIGEN PARTICLES

摘要

The present invention relates to methods for the direct determination of the antigen content of products comprising adjuvant-coupled-antigen particles. Specifically, the present invention relates to a method for determining the antigen content of a product comprising antigen-coupled-adjuvant particles, the method comprises the steps of: a) contacting the product comprising antigen-coupled-adjuvant particles with immunoglobulin molecules capable of recognizing the antigen under conditions allowing antigen-immunoglobulin binding; b) providing a surface with the antigen, without the adjuvant, immobilized thereon; c) contacting the immunoglobulin contacted product of step (a) with the surface of step (b) under conditions allowing antigen-immunoglobulin binding; d) removing non-bound immunoglobulin molecules and antigen-coupled-adjuvant particles bound immunoglobulin molecules; e) detecting antigen-bound immunoglobulin molecules thereby determining the antigen content of a product comprising antigen-coupled-adjuvant particles.