发明名称 |
METHODS FOR CONJUGATING NUCLEIC ACIDS WITH SMALL MOLECULES |
摘要 |
A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5′-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile. |
申请公布号 |
US2012177573(A1) |
申请公布日期 |
2012.07.12 |
申请号 |
US201113188989 |
申请日期 |
2011.07.22 |
申请人 |
WANG TZU-PIN;CIOU YI-JHANG;KAOHSIUNG MEDICAL UNIVERSITY |
发明人 |
WANG TZU-PIN;CIOU YI-JHANG |
分类号 |
A61K49/00;A61K31/7105;A61K48/00;C07D495/04;C07H1/00;C07K1/107;G01N33/53 |
主分类号 |
A61K49/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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