发明名称 |
BINDING-INDUCED HAIRPIN DETECTION SYSTEM |
摘要 |
<p>This application relates to a binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.</p> |
申请公布号 |
EP2473631(A1) |
申请公布日期 |
2012.07.11 |
申请号 |
EP20100811062 |
申请日期 |
2010.08.30 |
申请人 |
THE GOVERNORS OF THE UNIVERSITY OF ALBERTA |
发明人 |
LE, XIAOCHUN;ZHANG, HONGQUAN;LI, XING-FANG |
分类号 |
C12Q1/68;C40B30/04;G01N33/53;G01N33/542 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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