METHOD FOR RECOVERING PURE CULTURE OF TUBERCULOSIS MYCOBACTERIA

摘要

FIELD: medicine. ^ SUBSTANCE: pieces of the affected organs (a biomaterial) are grinded to prepare a suspension. The prepared suspension is filled with a solution containing 4-6% of citric acid and 3% of hydrogen peroxide in the ratio 1:1 to the biomaterial suspension and kept for 30-60 minutes at room temperature to be thereafter processed in a solution containing 4-5% of succinic acid and 3% of hydrogen peroxide in the ratio to the suspension 1:1. It is kept for 30-60 minutes and then neutralised with 5-10% ammonia to pH 7.0. It is followed by decantation of the supernatant, and the Lowenstein-Jensen agar medium is inoculated with the precipitation. ^ EFFECT: invention allows reducing mycobacteria recovery time. ^ 1 tbl, 1 ex