Nucleic acid amplification primers for PCR-based clonality studies of BCL2-IGH rearrangements

摘要

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a V²-J² TCRB rearrangement, and optionally a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V»-J» IGL rearrangement, a D²-J² TCRB rearrangement, a V³-J³ TCRG rearrangement, a V´-J´ TCRD rearrangement, a D´-D´ TCRD rearrangement, a D´-J´ TCRD rearrangement, a V´-D´ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-ICrH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.