| 摘要 |
A means for feeder-free culture which can sufficiently maintain the undifferentiation potency of pluripotent stem cells such as iPS cells even without using heterologously derived cells or proteins, is provided. Disclosed are a composition for pluripotent stem cell culture containing activin (preferably, activin A); a medium for pluripotent stem cell culture containing the composition; a method for culturing pluripotent stem cells for proliferating or establishing pluripotent stem cells such as iPS cells (preferably, mammalian iPS cells, and particularly preferably, human iPS cells) while maintaining an undifferentiated condition of the pluripotent stem cells, the method including performing the culture in the presence of activin A; and a method for preparing a pluripotent stem cell clonal population in an undifferentiated condition, the method including culturing undifferentiated pluripotent stem cells such as iPS cells (preferably, mammalian iPS cells, and particularly preferably, human iPS cells) in the presence of activin.
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