METHOD FOR CULTURE OF ENTOMOPATHOGENIC NEMATODE RHABDITIS BLUMI

摘要

PURPOSE: A method for culturing entomopathogenic nematode Rhabditis blumi is provided to enhance proliferation of Rhabditis blumi. CONSTITUTION: A method for producing entomopathogenic nematode Rhabditis blumi comprises a step of culturing the Rhabditis blumi in a medium containing symbiotic bacterium of Providencia vermicola, Flavobacterium sp., or Alcaligenes faecalis. The medium contains Providancia vermicola, Flavobacterium sp. and Alcaligenes faecalis. The concentration of the Providancia vermicola in the medium is 1 x 109cfu/ml to 1 x 1011cfu/ml. The medium also contains yeast extract, cholesterol, vegetable oil, soybean hydrolysate, or sodium chloride.