发明名称 |
METHODS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF SUGAR CANE |
摘要 |
The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an Agrobacterium inoculation suspension, said Agrobacterium comprising a nucleic acid of interest, to obtain an Agrobacterium-inoculated sugar cane tissue or cell thereof; b) co-cultivating said Agrobacterium-inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said Agrobacterium-inoculated-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants. |
申请公布号 |
US2012156673(A1) |
申请公布日期 |
2012.06.21 |
申请号 |
US201013378497 |
申请日期 |
2010.06.24 |
申请人 |
DE LUCCA PAULO CEZAR;DONG SHUJIE;GEIJSKES ROBERT JASON CHRISTOPHER;DUNDER ERIK MARTIN;SAINZ MANUEL BENITO |
发明人 |
DE LUCCA PAULO CEZAR;DONG SHUJIE;GEIJSKES ROBERT JASON CHRISTOPHER;DUNDER ERIK MARTIN;SAINZ MANUEL BENITO |
分类号 |
C12Q1/68;C12N15/82 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|