O-PHOSPHOSERINE SULFHYDRYLASE MUTANTS AND METHOD FOR PRODUCTION OF CYSTEINE USING THE SAME
摘要
Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis-derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction.
申请公布号
WO2012053777(A3)
申请公布日期
2012.06.21
申请号
WO2011KR07678
申请日期
2011.10.14
申请人
CJ CHEILJEDANG CORPORATION;SHIN, SOO AN;CHANG, JIN SOOK;UM, HYE WON;JO, JAE HYUN;SONG, BYEONG CHEOL;LEE, KYOUNG MIN
发明人
SHIN, SOO AN;CHANG, JIN SOOK;UM, HYE WON;JO, JAE HYUN;SONG, BYEONG CHEOL;LEE, KYOUNG MIN